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Isolation and investigation of biological potential of endophytic fungi in Eleochararis dulcis

Viet Nguyen -Thi 1, 2
Truc -Thanh Tran 1, 2
Tam Dinh Le Vo 1, 2
Trung-Dung Huynh-Ngoc 3
Thu Huynh 1, 2, *
  1. Department of Biotechnology, Faculty of Chemical Engineering, Ho Chi Minh University of Technology
  2. Vietnam National University Ho Chi Minh City, Vietnam
  3. Faculty of Pharmacy and Nursing, Tay Do University, Vietnam
Correspondence to: Thu Huynh, Department of Biotechnology, Faculty of Chemical Engineering, Ho Chi Minh University of Technology; Vietnam National University Ho Chi Minh City, Vietnam. Email: [email protected].
Volume & Issue: Vol. 8 No. 2 (2025) | Page No.: 2558-2564 | DOI: 10.32508/stdjet.v8i2.1424
Published: 2025-06-30

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This article is published with open access by Viet Nam National University, Ho Chi Minh City, Viet Nam. This article is distributed under the terms of the Creative Commons Attribution License (CC-BY 4.0) which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited. 

Abstract

The grass is a common wild plant in the river delta region of the Southwest region of Vietnam. They grow abundantly and flourish in saline-acidic areas. Native grasses  interact with their endogenous microorganisms. However, there have not been many studies on this subject, especially the grass in Vietnam.

From the grass, the study isolated 3 endophytic fungi from the root. The results showed that the fungal strain with the highest P-decomposing ability was NR3 with a PO43- content of 696.74 mg/L. All 3 strains were able to grow and develop, but the decomposing ability was not observed. In addition, all 3 fungal strains were able to synthesize N but the results were quite low. The ability to synthesize the plant hormone IAA in PDB medium with/without L-Tryptophan was investigated and quantified by spectrophotometry with Salkowski reagent. The results showed that in the environment without L-Tryptophan supplementation, strain NR3 had the highest IAA synthesis capacity with a total IAA content of 12.06 mg/L. In the environment with L-Tryptophan supplementation, the amount of IAA produced was higher, in which strain NR1 had an extracellular IAA content of 24.62 mg/L. The research results provided initial data on the biological capacity of endophytic fungi in the roots of reed grass, creating the premise for further studies.

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